The best Side of attune flow cytometer
The best Side of attune flow cytometer
Blog Article
Data-enriched characterization of cell populations. Morphological info from pictures can add into the richness of flow cytometry data. By way of example, the determine exhibits an in any other case regular apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in Each and every populace to expose morphologically unique functions. These insights couldn't have been obtained from multiplex staining on your own.
As samples are acquired to the Attune CytPix Flow Cytometer, the high-pace brightfield digicam captures and stores images of detected gatherings at a price nearly 6,000 images for each 2nd, with regards to the flow rate and graphic size. For greater flexibility, Attune Cytometric Application enables you to modify the impression capture frequency as essential and choose a particular gate for image capture, if required.
Incorporate acoustic concentrating technology with superior-parameter analysis for flexible spectral unmixing and traditional workflows.
Find new insights into your experimental details by visualizing populations employing merged fluorescence and morphology info. Our program interface wizards allow downsampling, concatenation (merged documents) and dimensionality equipment which are developed in to the program for an intuitive, streamlined facts Examination working experience.
"An image isn't pretty worth a thousand dots, nonetheless it actually helps to see pictures. It is great to find out pictures that correlate with staining. By way of example, AnnexinV-constructive cells that happen to be morphologically distinct than wholesome cells, or CD14+ monocytes that happen to be larger sized and much more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a highly skilled user has gated singlets confidently. Just after assessing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of much more than four% aggregates.
Optimizing gating method. Even robust manual singlet gating is error-vulnerable and remains a subjective choice position in Nearly all flow cytometry assays. Imaging can be used to substantiate and change gates to incorporate only single cells of interest.
Our scientists will style panels for you as repeatedly you would like—cost-free, no products buy needed.
Early signs of this contamination experienced originally been dismissed as particles. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also means that you can use morphological features to find out fascinating subpopulations that would not be obvious from flow cytometry knowledge by yourself.
Possibly most significantly, these events include cells of clearly various phenotypes which could lead to incorrect conclusions concerning double favourable situations (particularly in rare populations).
Sound waves permit exact mobile alignment, resulting in exceptional details top quality and ~10X speedier sample flow fees.
Software Observe: Automatic picture Evaluation cuts down user-to-consumer variability in flow cytometry gating procedures
Leverage the power of advanced technological innovation and innovation to speed up your discoveries. Trust during the Attune Flow Cytometer household to deliver the trustworthiness, speed, check here and accuracy that set your work aside.
Versatile payment program from Agilent Money Remedies allow you to have the essential instrumentation you need for immediate undertaking, or to improve your lab.
Gating on these prolonged parameters lets you promptly and accurately discover populations of desire and make sure gating approach with little if any manual evaluate. The Attune program gives usage of point out-of-the-artwork machine learning Assessment resources inside of an expertly designed consumer interface, rendering it exceptionally simple to use.
The Attune Xenith cytometer is a substantial-parameter spectral analyzer, delivering both equally spectral unmixing and conventional flow abilities for thorough and versatile cell Assessment.
Agilent Worth Guarantee assures you at the very least ten years of instrument use in the date you buy. Normally, We'll credit you Together with the residual value of the process towards an upgraded product.
Right here, we exhibit that by gating methods making use of graphic-dependent quantitative parameters can distinguish interacting cells from coincident situations to extra accurately examine interacting cells.